Hotspot

ndhF

SSC · 112091-114313 · 2223 bp

Back to Lycium

Pi 0.00225
MarkerSeek score 32.90
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2223
variable_sites
38
pi
0.00225
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACAGGAACAAGAGGGATCCA TGACGCCTTTCAAAAGAGGT 50.0 45.0 58.0 57.7 2497-2520 bp 0.576923 62.3
ndhF_p2 AGGGTGGAAAGTGAGGAAGA TGACGCCTTTCAAAAGAGGT 50.0 45.0 57.9 57.7 2562-2585 bp 0.576923 61.6
ndhF_p3 CGGACAAAATCGACGAAACG TGACGCCTTTCAAAAGAGGT 50.0 45.0 58.2 57.7 2423-2446 bp 0.576923 61.5
ndhF_p4 GATCCGGACAAAATCGACGA TGACGCCTTTCAAAAGAGGT 50.0 45.0 58.1 57.7 2442 bp 0.057692 38.9
ndhF_p5 TCCGGACAAAATCGACGAAA TGACGCCTTTCAAAAGAGGT 45.0 45.0 57.8 57.7 2440 bp 0.057692 38.4