Hotspot

clpP1

LSC · 72405-74439 · 2035 bp

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Pi 0.00237
MarkerSeek score 53.40
Primer available yes
Label clpP1

Feature Metadata

label_name
clpP1
feature_type
gene
region
LSC
length_bp
2035
variable_sites
23
pi
0.00237
unique_haplotype_count
35
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP1_p1 GATTTGCGCGAAATCCATGT ACGTTTCCACCTCAAAGTGA 45.0 45.0 57.8 57.3 2087-2123 bp 1.0 75.7
clpP1_p2 CAAATCACAGATTTGCGCGA ACGTTTCCACCTCAAAGTGA 45.0 45.0 57.8 57.3 2096-2132 bp 1.0 75.2
clpP1_p3 TCAAATCACAGATTTGCGCG ACGTTTCCACCTCAAAGTGA 45.0 45.0 57.8 57.3 2097-2133 bp 1.0 75.2
clpP1_p4 GATTTGCGCGAAATCCATGT ACGTTTCCACCTCAAAGTGAA 45.0 42.9 57.8 58.0 2087-2123 bp 1.0 73.3
clpP1_p5 CAAATCACAGATTTGCGCGA ACGTTTCCACCTCAAAGTGAA 45.0 42.9 57.8 58.0 2096-2132 bp 1.0 72.8