Hotspot

ccsA

SSC · 116222-117163 · 942 bp

Pi 0.00234

MarkerSeek score 31.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 942
variable_sites: 12
pi: 0.00234
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCATACCGTCTTCTAAAAAGG	ATTTGGAATAACTTGTCTTGAAT	45.5	26.1	57.1	52.6	1072-1073 bp	1.0	41.0
ccsA_p2	GGCATACCGTCTTCTAAAAAGGA	ATTTGGAATAACTTGTCTTGAAT	43.5	26.1	58.5	52.6	1072-1073 bp	1.0	41.0
ccsA_p3	TGAATTCAATTCCCGATTTCCA	ATTTGGAATAACTTGTCTTGAAT	36.4	26.1	56.3	52.6	1033-1034 bp	1.0	41.0
ccsA_p4	GCATACCGTCTTCTAAAAAGGA	ATTTGGAATAACTTGTCTTGAAT	40.9	26.1	56.2	52.6	1071-1072 bp	1.0	41.0
ccsA_p5	TGAATTCAATTCCCGATTTCCAT	ATTTGGAATAACTTGTCTTGAAT	34.8	26.1	56.8	52.6	1033-1034 bp	1.0	41.0