Hotspot

rpl36-rps8

LSC · 82086-82531 · 446 bp

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Pi 0.00878
MarkerSeek score 45.30
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
446
variable_sites
27
pi
0.00878
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 ACTATAATTCGTCCCCGCCT GAGAGGCTCGACTAGAAGGA 50.0 55.0 58.3 57.7 573-625 bp 1.0 79.8
rpl36-rps8_p2 ACTATAATTCGTCCCCGCCT TCCGCGAATTTTAGGTGGAA 50.0 45.0 58.3 57.5 638-690 bp 1.0 78.1
rpl36-rps8_p3 ACTATAATTCGTCCCCGCCT TTCCGCGAATTTTAGGTGGA 50.0 45.0 58.3 57.5 639-691 bp 1.0 78.1
rpl36-rps8_p4 ACTATAATTCGTCCCCGCCT AGAGGCTCGACTAGAAGGAA 50.0 50.0 58.3 57.2 572-624 bp 1.0 76.0
rpl36-rps8_p5 ACTATAATTCGTCCCCGCCT TCCGCGAATTTTAGGTGGAAT 50.0 42.9 58.3 58.0 638-690 bp 1.0 73.9