Hotspot

petA-psbJ

LSC · 65257-66333 · 1077 bp

Back to Lycium

Pi 0.00407
MarkerSeek score 57.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1077
variable_sites
28
pi
0.00407
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1117-1147 bp 1.0 81.7
petA-psbJ_p2 TCGAGAAGGTTCAATTGGCC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1119-1149 bp 1.0 81.7
petA-psbJ_p3 AGAAGGTTCAATTGGCCGAA ATTCCGGATTGGGTTCATCC 45.0 50.0 57.7 57.6 1116-1146 bp 1.0 80.8
petA-psbJ_p4 ACGTGTCCAAGGACTTTTGT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.6 57.6 1196-1226 bp 1.0 79.7
petA-psbJ_p5 TCCATTACGTGTCCAAGGAC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.2 57.6 1202-1232 bp 1.0 76.9