Hotspot

rbcL-accD

LSC · 58661-59454 · 794 bp

Back to Lycium

Pi 0.00428
MarkerSeek score 42.50
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
794
variable_sites
19
pi
0.00428
unique_haplotype_count
27
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 ACTACAGTTCGGTGGAGGAA ACTGGAACCCTCGTTTTCAC 50.0 50.0 58.0 58.0 1594-1654 bp 1.0 84.1
rbcL-accD_p2 ACTACAGTTCGGTGGAGGAA CAATAGGACCAAGACTGCCC 50.0 55.0 58.0 58.0 1136-1178 bp 1.0 83.8
rbcL-accD_p3 AGAATTGGGTGTTCCGATCG ACTGGAACCCTCGTTTTCAC 50.0 50.0 58.0 58.0 2017-2077 bp 1.0 83.3
rbcL-accD_p4 AGAATTGGGTGTTCCGATCG CAATAGGACCAAGACTGCCC 50.0 55.0 58.0 58.0 1559-1601 bp 1.0 82.9
rbcL-accD_p5 GCATGACTACTTAACGGGGG ACTGGAACCCTCGTTTTCAC 55.0 50.0 58.1 58.0 1993-2053 bp 0.980769 82.3