Hotspot

ndhC-trnV-UAC

LSC · 52229-53360 · 1132 bp

Back to Lycium

Pi 0.00465
MarkerSeek score 58.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1132
variable_sites
36
pi
0.00465
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTGCTAATCGGGGCTAACAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1197-1297 bp 1.0 84.6
ndhC-trnV-UAC_p2 CCCCGGAAATGAAAAATGCC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1178-1278 bp 0.980769 83.8
ndhC-trnV-UAC_p3 GCTAACACCCCGGAAATGAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1185-1285 bp 1.0 83.5
ndhC-trnV-UAC_p4 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1200-1300 bp 1.0 82.1
ndhC-trnV-UAC_p5 CCCCGGAAATGAAAAATGCC GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.0 57.5 1177-1277 bp 0.980769 81.0