Hotspot

trnS-GGA-rps4

LSC · 46796-47132 · 337 bp

Back to Lycium

Pi 0.0077
MarkerSeek score 51.70
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
337
variable_sites
15
pi
0.0077
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 AGGCTTTTGTTTACCGAGGG TTTGACCCTTCACCCATTCC 50.0 50.0 58.1 57.7 485-507 bp 1.0 83.3
trnS-GGA-rps4_p2 AGGCTTTTGTTTACCGAGGG GACCCTTCACCCATTCCAAT 50.0 50.0 58.1 57.5 482-504 bp 1.0 81.4
trnS-GGA-rps4_p3 GGCTTTTGTTTACCGAGGGT TTTGACCCTTCACCCATTCC 50.0 50.0 58.4 57.7 484-506 bp 1.0 80.9
trnS-GGA-rps4_p4 ATGTAGGAGAGATGGCCGAG TTTGACCCTTCACCCATTCC 55.0 50.0 58.4 57.7 537-559 bp 1.0 80.8
trnS-GGA-rps4_p5 CACTCGGTTTTGGGGTCATA TTTGACCCTTCACCCATTCC 50.0 50.0 57.5 57.7 563-585 bp 1.0 79.8