Hotspot

psbM-trnD-GUC

LSC · 30416-31490 · 1075 bp

Back to Lycium

Pi 0.00308
MarkerSeek score 52.50
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1075
variable_sites
20
pi
0.00308
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGTAGAAAAGCGGTAGGAACT CCCGTATTTCCCTGGGATTG 42.9 55.0 56.4 58.0 1005-1249 bp 1.0 64.4
psbM-trnD-GUC_p2 AGTAGAAAAGCGGTAGGAACT TCCCGTATTTCCCTGGGATT 42.9 50.0 56.4 58.1 1006-1250 bp 1.0 63.7
psbM-trnD-GUC_p3 AAGTAGAAAAGCGGTAGGAACT CCCGTATTTCCCTGGGATTG 40.9 55.0 57.1 58.0 1006-1250 bp 1.0 62.2
psbM-trnD-GUC_p4 AGAAAAGCGGTAGGAACTAGA CCCGTATTTCCCTGGGATTG 42.9 55.0 56.1 58.0 1002-1246 bp 1.0 62.1
psbM-trnD-GUC_p5 AAGTAGAAAAGCGGTAGGAACT TCCCGTATTTCCCTGGGATT 40.9 50.0 57.1 58.1 1007-1251 bp 1.0 61.5