Hotspot

rpoC1-rpoB

LSC · 23856-23881 · 26 bp

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Pi 0.00992
MarkerSeek score 54.40
Primer available yes
Label rpoC1-rpoB

Feature Metadata

label_name
rpoC1-rpoB
feature_type
igs
region
LSC
length_bp
26
variable_sites
2
pi
0.00992
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-rpoB_p1 TGGCCCAAGCACTTATTTGT TTGCTCGTTCGAGAACTACG 45.0 50.0 58.0 58.0 187-192 bp 1.0 84.2
rpoC1-rpoB_p2 TTTTAGTGGCCCAAGCACTT TTGCTCGTTCGAGAACTACG 45.0 50.0 57.9 58.0 193-198 bp 1.0 83.5
rpoC1-rpoB_p3 TGGCCCAAGCACTTATTTGT CCCGAAGATGCTCCAGAATC 45.0 55.0 58.0 58.1 214-219 bp 1.0 83.0
rpoC1-rpoB_p4 AGGATTTTAGTGGCCCAAGC TTGCTCGTTCGAGAACTACG 50.0 50.0 58.1 58.0 197-202 bp 1.0 82.9
rpoC1-rpoB_p5 TTTTAGTGGCCCAAGCACTT CCCGAAGATGCTCCAGAATC 45.0 55.0 57.9 58.1 220-225 bp 1.0 82.3