Hotspot

atpH-atpI

LSC · 13614-14836 · 1223 bp

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Pi 0.00485
MarkerSeek score 57.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1223
variable_sites
39
pi
0.00485
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TACCTGTCATGCTCCTTGGA 45.0 50.0 58.0 58.0 1298-1389 bp 1.0 85.6
atpH-atpI_p2 TACCCTCTACAGCTTGACCC TACCTGTCATGCTCCTTGGA 55.0 50.0 58.1 58.0 1381-1472 bp 1.0 84.7
atpH-atpI_p3 GCGATACCCTCTACAGCTTG TACCTGTCATGCTCCTTGGA 55.0 50.0 57.9 58.0 1385-1476 bp 1.0 84.7
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA TACCTGTCATGCTCCTTGGA 45.0 50.0 58.2 58.0 1299-1390 bp 1.0 84.5
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC TACCTGTCATGCTCCTTGGA 50.0 50.0 57.8 58.0 1313-1404 bp 1.0 84.2