Hotspot

trnK-UUU-rps16

LSC · 4386-5075 · 690 bp

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Pi 0.00522
MarkerSeek score 59.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
690
variable_sites
31
pi
0.00522
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCAGTCGTGGTCTTCCAAAC TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 816-916 bp 1.0 81.3
trnK-UUU-rps16_p2 AAAAGACACTAGCCGCACTT TTGAAAAAGGGGCTCAACCT 45.0 45.0 57.7 57.5 752-852 bp 1.0 81.3
trnK-UUU-rps16_p3 CAGTCGTGGTCTTCCAAACT TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 815-915 bp 1.0 81.2
trnK-UUU-rps16_p4 TCAGTCGTGGTCTTCCAAAC AAAAGGGGCTCAACCTACAG 50.0 50.0 57.8 57.4 812-912 bp 1.0 80.4
trnK-UUU-rps16_p5 CAGTCGTGGTCTTCCAAACT AAAAGGGGCTCAACCTACAG 50.0 50.0 57.8 57.4 811-911 bp 1.0 80.3