Hotspot

rpoA-rps11

LSC · 122960-123019 · 60 bp

Back to Lutheria

Pi 0.0167

MarkerSeek score 62.10

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 1
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	TGCTACTTCCTCTCGAACCA	AGAGATGCAGCATTACGAGC	50.0	50.0	58.1	58.1	195 bp	1.0	78.7
rpoA-rps11_p2	TCGAGTGGATCCTGCTACTT	AGAGATGCAGCATTACGAGC	50.0	50.0	57.9	58.1	207 bp	1.0	78.1
rpoA-rps11_p3	TTCGAGTGGATCCTGCTACT	AGAGATGCAGCATTACGAGC	50.0	50.0	57.9	58.1	208 bp	1.0	78.1
rpoA-rps11_p4	TGTTCGAGTGGATCCTGCTA	AGAGATGCAGCATTACGAGC	50.0	50.0	58.2	58.1	210 bp	1.0	78.1
rpoA-rps11_p5	GTTCGAGTGGATCCTGCTAC	AGAGATGCAGCATTACGAGC	55.0	50.0	57.8	58.1	209 bp	1.0	77.6