Hotspot

psaJ-rpl33

LSC · 111020-111533 · 514 bp

Back to Lutheria

Pi 0.0118
MarkerSeek score 64.70
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
514
variable_sites
6
pi
0.0118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTGGGTCTTTAGCGGGTCTA CCCGCGATTCCTTATTGACA 50.0 50.0 58.1 58.1 673-674 bp 1.0 80.4
psaJ-rpl33_p2 TATTCCCAGATGCCCTGTCA CCCGCGATTCCTTATTGACA 50.0 50.0 58.1 58.1 634-635 bp 1.0 80.1
psaJ-rpl33_p3 CGTGGCACCTGTGTTAACTA CCCGCGATTCCTTATTGACA 50.0 50.0 57.8 58.1 702-703 bp 1.0 79.6
psaJ-rpl33_p4 ATGGTTTGGGTCTTTAGCGG CCCGCGATTCCTTATTGACA 50.0 50.0 58.2 58.1 678-679 bp 1.0 79.6
psaJ-rpl33_p5 TGGGTCTTTAGCGGGTCTAT CCCGCGATTCCTTATTGACA 50.0 50.0 57.8 58.1 672-673 bp 1.0 79.5