Hotspot

petA-psbJ

LSC · 106302-107434 · 1133 bp

Back to Lutheria

Pi 0.0132

MarkerSeek score 60.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1133
variable_sites: 15
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.9	58.0	1259 bp	1.0	80.2
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.9	58.0	1257 bp	1.0	80.2
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	50.0	55.0	57.9	57.6	1258 bp	1.0	77.3
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.7	58.0	1266 bp	1.0	75.1
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.7	58.0	1264 bp	1.0	75.1