Hotspot

accD-psaI

LSC · 101629-102539 · 911 bp

Back to Lutheria

Pi 0.0177
MarkerSeek score 55.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
911
variable_sites
16
pi
0.0177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TGGTTCCTATGGACCCCATT AATAGACCTGCGATCCCGTA 50.0 50.0 58.0 58.0 2237-2251 bp 1.0 81.6
accD-psaI_p2 TGGTTCCTATGGACCCCATT ATAGACCTGCGATCCCGTAA 50.0 50.0 58.0 58.0 2236-2250 bp 1.0 81.6
accD-psaI_p3 TGGTTCCTATGGACCCCATT CCTGCGATCCCGTAAAAAGA 50.0 50.0 58.0 58.0 2231-2245 bp 1.0 81.5
accD-psaI_p4 GACAGTACCCGATGGTTCAC AATAGACCTGCGATCCCGTA 55.0 50.0 58.0 58.0 1758 bp 0.5 59.9
accD-psaI_p5 GACAGTACCCGATGGTTCAC ATAGACCTGCGATCCCGTAA 55.0 50.0 58.0 58.0 1757 bp 0.5 59.9