Hotspot

ndhC-trnV-UAC

LSC · 93424-94224 · 801 bp

Back to Lutheria

Pi 0.00749
MarkerSeek score 63.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
801
variable_sites
6
pi
0.00749
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 938 bp 1.0 78.7
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAACTCC GAGCCCAATGCAACAAACAA 55.0 45.0 58.0 57.8 1025 bp 1.0 78.3
ndhC-trnV-UAC_p3 CGGGGCTAAAACTCCTGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 933 bp 1.0 77.4
ndhC-trnV-UAC_p4 CGGGGCTAAAACTCCTGAAA GAGCCCAATGCAACAAACAA 50.0 45.0 57.8 57.8 1020 bp 1.0 77.0
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAACTCCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 937 bp 1.0 76.2