Hotspot

petN-psbM

LSC · 71242-72461 · 1220 bp

Back to Lutheria

Pi 0.00902
MarkerSeek score 52.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1220
variable_sites
11
pi
0.00902
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTATGGGGAAGGAGTGGAC TCTACGTAAAAACAGTCAGTCA 55.0 36.4 58.1 55.1 1277-1289 bp 1.0 40.8
petN-psbM_p2 GTATGGGGAAGGAGTGGACT TCTACGTAAAAACAGTCAGTCA 55.0 36.4 58.1 55.1 1276-1288 bp 1.0 40.8
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TCTACGTAAAAACAGTCAGTCA 50.0 36.4 58.2 55.1 1335-1347 bp 1.0 40.7
petN-psbM_p4 TATGGGGAAGGAGTGGACTC TCTACGTAAAAACAGTCAGTCA 55.0 36.4 57.8 55.1 1275-1287 bp 1.0 40.7
petN-psbM_p5 GTATGGGGAAGGAGTGGACT ACGTAAAAACAGTCAGTCAA 55.0 35.0 58.1 53.0 1273-1285 bp 1.0 40.7