Hotspot

trnS-GCU-trnG-UCC

LSC · 49501-50680 · 1180 bp

Back to Lutheria

Pi 0.0161
MarkerSeek score 68.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1180
variable_sites
17
pi
0.0161
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GAGAAAGCGGAAAGAGAGGG AACCGATGGACCCCTTAACT 55.0 50.0 58.0 58.3 1227-1347 bp 1.0 82.2
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA AACCGATGGACCCCTTAACT 45.0 50.0 58.0 58.3 1172-1292 bp 1.0 82.1
trnS-GCU-trnG-UCC_p3 GAGAAAGCGGAAAGAGAGGG AAACCGATGGACCCCTTAAC 55.0 50.0 58.0 57.5 1228-1348 bp 1.0 80.9
trnS-GCU-trnG-UCC_p4 ATTAGCAATCCGCCGCTTTA AAACCGATGGACCCCTTAAC 45.0 50.0 58.0 57.5 1173-1293 bp 1.0 80.8
trnS-GCU-trnG-UCC_p5 GAGAAAGCGGAAAGAGAGGG ACCGATGGACCCCTTAACTA 55.0 50.0 58.0 57.4 1226-1346 bp 1.0 80.5