Hotspot

ycf1

SSC · 108008-113335 · 5328 bp

Back to Lupinus

Pi 0.0253

MarkerSeek score 56.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 289
pi: 0.0253
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCTATACGCGCTCAAAGGC	CGGGGATAACCAGAAAAGGG	50.0	55.0	58.2	57.9	699-702 bp	1.0	83.2
ycf1_p2	ACAACAAACGGATTTTCGGC	CGGGGATAACCAGAAAAGGG	45.0	55.0	57.9	57.9	735-738 bp	0.8	74.0
ycf1_p3	ACAACAAACGGATTTTCGGC	ATCTGTGCCAAGGTTTCAGG	45.0	50.0	57.9	58.1	617 bp	0.8	73.1
ycf1_p4	ACAACAAACGGATTTTCGGC	AAGGCGATTTCGGGGATAAC	45.0	50.0	57.9	58.0	745-748 bp	0.6	65.5
ycf1_p5	ATCTATACGCGCTCAAAGGC	AAGGCGATTTCGGGGATAAC	50.0	50.0	58.2	58.0	709-712 bp	0.6	64.9