Hotspot

clpP

LSC · 68130-70117 · 1988 bp

Back to Lupinus

Pi 0.0156

MarkerSeek score 59.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1988
variable_sites: 65
pi: 0.0156
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GTTGGCATGGTGAGTCGTAT	CATAGAGAGCCATCGAACCG	50.0	55.0	58.0	57.9	2727-2795 bp	1.0	83.9
clpP_p2	GTTGGCATGGTGAGTCGTAT	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2791-2859 bp	1.0	83.8
clpP_p3	ACCTCGCCGATACTGGAATA	CATAGAGAGCCATCGAACCG	50.0	55.0	58.0	57.9	2902-2970 bp	1.0	83.7
clpP_p4	CTGAACGAGTCGCACATACA	CATAGAGAGCCATCGAACCG	50.0	55.0	58.0	57.9	2848-2916 bp	1.0	83.7
clpP_p5	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2912-2980 bp	1.0	83.6