Hotspot

rpl32-ndhF

SSC · 123700-124181 · 482 bp

Back to Lupinus

Pi 0.0288

MarkerSeek score 68.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 482
variable_sites: 29
pi: 0.0288
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TCCTAAGAGCAGCGTGTCTA	GGAAAAAGTAGGAGCCCCAC	50.0	55.0	57.9	58.2	1040-1075 bp	1.0	84.2
rpl32-ndhF_p2	TCCTAAGAGCAGCGTGTCTA	GTGGGAAAAAGTAGGAGCCC	50.0	55.0	57.9	58.2	1043-1078 bp	1.0	84.1
rpl32-ndhF_p3	CGAAGATCAAATGCCATGCC	GGAAAAAGTAGGAGCCCCAC	50.0	55.0	57.9	58.2	1097-1132 bp	1.0	83.9
rpl32-ndhF_p4	GAAGATCAAATGCCATGCCG	GGAAAAAGTAGGAGCCCCAC	50.0	55.0	57.9	58.2	1096-1131 bp	1.0	83.9
rpl32-ndhF_p5	GAAGATCAAATGCCATGCCG	GTGGGAAAAAGTAGGAGCCC	50.0	55.0	57.9	58.2	1099-1134 bp	1.0	83.9