Hotspot

ycf1-rps15

SSC · 113336-113787 · 452 bp

Back to Lupinus

Pi 0.0347

MarkerSeek score 72.10

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 452
variable_sites: 34
pi: 0.0347
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ATTGAGCTGGAGAGGTTCCT	TCCTTTCGGTGCAATTCCAA	50.0	45.0	58.0	58.0	1328-1340 bp	0.8	77.0
ycf1-rps15_p2	ATTGAGCTGGAGAGGTTCCT	CGGATTTGTGACTCCCGAAT	50.0	50.0	58.0	58.0	1460-1472 bp	0.8	76.6
ycf1-rps15_p3	ATTGAGCTGGAGAGGTTCCT	TAATCGTTGCCGTTTTCCCA	50.0	45.0	58.0	58.1	1380-1392 bp	0.8	76.2
ycf1-rps15_p4	ATTGAGCTGGAGAGGTTCCT	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	1653-1665 bp	0.8	76.0
ycf1-rps15_p5	ATTGAGCTGGAGAGGTTCCT	CCAACGTGTTACATAGGGCA	50.0	50.0	58.0	57.9	1788-1800 bp	0.8	75.3