Hotspot

trnR-ACG-trnN-GUU

IRb · 106953-107527 · 575 bp

Back to Lupinus

Pi 0.0153

MarkerSeek score 69.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 575
variable_sites: 19
pi: 0.0153
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	715-716 bp	1.0	80.5
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	752-753 bp	1.0	79.8
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	756-757 bp	1.0	79.6
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	AGCTCGAATTTCCCGTTGTT	55.0	45.0	57.8	58.1	727-728 bp	1.0	79.6
trnR-ACG-trnN-GUU_p5	TTTTAGGGTACTCCGGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.7	58.0	769-770 bp	1.0	79.3