Hotspot

petA-psbJ

LSC · 60523-61691 · 1169 bp

Back to Lupinus

Pi 0.0244

MarkerSeek score 63.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1169
variable_sites: 64
pi: 0.0244
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTGTGATAGGCCCTGTTCC	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	2373-2388 bp	1.0	82.0
petA-psbJ_p2	CTTGTGATAGGCCCTGTTCC	ATGTATTTGGAAGTCCCCGC	55.0	50.0	58.0	57.9	2324-2339 bp	1.0	82.0
petA-psbJ_p3	CTTGTGATAGGCCCTGTTCC	ATTCATTGCGGGTTGGCTAT	55.0	45.0	58.0	57.9	2368-2383 bp	1.0	81.8
petA-psbJ_p4	CTTGTGATAGGCCCTGTTCC	CATTGCGGGTTGGCTATTTG	55.0	50.0	58.0	58.1	2365-2380 bp	1.0	81.8
petA-psbJ_p5	CTTGTGATAGGCCCTGTTCC	TCAGCACGGGTTTAGCTTAC	55.0	50.0	58.0	57.9	2345-2360 bp	1.0	81.7