Hotspot

ycf4-cemA

LSC · 58386-58697 · 312 bp

Back to Lupinus

Pi 0.035

MarkerSeek score 65.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 23
pi: 0.035
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATAGGACAGGGTGCCATTC	CGTGTTTCCGGGTATTCCTT	55.0	50.0	58.0	57.9	665-682 bp	1.0	83.2
ycf4-cemA_p2	CATAGGACAGGGTGCCATTC	TCGTTGTGCGTCTTGATCAA	55.0	45.0	58.0	58.1	728-745 bp	1.0	82.9
ycf4-cemA_p3	GGGGGTCTCGAAAAACAAGT	CGTGTTTCCGGGTATTCCTT	50.0	50.0	58.0	57.9	1062-1079 bp	1.0	82.1
ycf4-cemA_p4	GGGGGTCTCGAAAAACAAGT	TCGTTGTGCGTCTTGATCAA	50.0	45.0	58.0	58.1	1125-1142 bp	1.0	81.8
ycf4-cemA_p5	GGGGGTCTCGAAAAACAAGT	TTCGTTGTGCGTCTTGATCA	50.0	45.0	58.0	58.1	1126-1143 bp	1.0	81.8