Hotspot

psaA-ycf3

LSC · 49309-50080 · 772 bp

Back to Lupinus

Pi 0.0146

MarkerSeek score 65.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 23
pi: 0.0146
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGCCCATTCCTCGAAAGAAG	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	718-922 bp	1.0	85.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	717-921 bp	1.0	85.2
psaA-ycf3_p3	TGCCCATTCCTCGAAAGAAG	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	724-928 bp	1.0	84.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	723-927 bp	1.0	84.9
psaA-ycf3_p5	TGCCCATTCCTCGAAAGAAG	ATCAGGCCGCTGAATATTGG	50.0	50.0	57.9	58.1	749-953 bp	1.0	84.3