Hotspot

trnR-UCU-atpA

LSC · 18036-18428 · 393 bp

Back to Lupinus

Pi 0.0224

MarkerSeek score 64.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 17
pi: 0.0224
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TGAAGGAGGCTCTTCAGGAA	47.6	50.0	57.3	58.0	436-488 bp	1.0	73.2
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	GGCTCTTCAGGAACAGATCG	47.6	55.0	57.3	58.1	429-481 bp	1.0	72.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	455-507 bp	1.0	72.6
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TTCACTGGAGAAGCAGAAGC	47.6	50.0	57.3	57.8	461-513 bp	1.0	71.9
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	AAGGAGGCTCTTCAGGAACA	47.6	50.0	57.3	58.3	434-486 bp	1.0	71.3