Hotspot

trnT-UGU-rps4

LSC · 14940-15225 · 286 bp

Back to Lupinus

Pi 0.0245

MarkerSeek score 65.20

Primer available yes

Label trnT-UGU-rps4

Feature Metadata

label_name: trnT-UGU-rps4
feature_type: igs
region: LSC
length_bp: 286
variable_sites: 15
pi: 0.0245
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-rps4_p1	CTCAGAGGTTAGAGCATCGC	CTGCGGGCCTTTTACTAGTT	55.0	50.0	57.9	57.9	413-430 bp	1.0	84.9
trnT-UGU-rps4_p2	CTCAGAGGTTAGAGCATCGC	AGCGCGATTGGTTTCTAAGT	55.0	45.0	57.9	57.9	449-466 bp	1.0	84.5
trnT-UGU-rps4_p3	ATGGTCATCGGTTCGATTCC	CTGCGGGCCTTTTACTAGTT	50.0	50.0	57.8	57.9	381-398 bp	1.0	84.3
trnT-UGU-rps4_p4	CTCAGAGGTTAGAGCATCGC	TTTGTTTGAAACGAGGCCCT	55.0	45.0	57.9	58.2	365-382 bp	1.0	84.3
trnT-UGU-rps4_p5	ATGGTCATCGGTTCGATTCC	AGCGCGATTGGTTTCTAAGT	50.0	45.0	57.8	57.9	417-434 bp	1.0	84.1