Hotspot

trnF-GAA-trnL-UAA

LSC · 13261-13682 · 422 bp

Back to Lupinus

Pi 0.0277

MarkerSeek score 66.50

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: LSC
length_bp: 422
variable_sites: 24
pi: 0.0277
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	CATTTCTACTCTTCCCGCCC	TTTCGCGTTGGGTTAGGAAA	55.0	45.0	58.0	58.0	1098-1109 bp	1.0	84.8
trnF-GAA-trnL-UAA_p2	TTTGAACTGGTGACACGAGG	TTTCGCGTTGGGTTAGGAAA	50.0	45.0	58.1	58.0	792-804 bp	1.0	84.6
trnF-GAA-trnL-UAA_p3	CATTTCTACTCTTCCCGCCC	GGGGATATGGCGAAATTGGT	55.0	50.0	58.0	58.0	1334-1345 bp	1.0	84.3
trnF-GAA-trnL-UAA_p4	TTTGAACTGGTGACACGAGG	GGGGATATGGCGAAATTGGT	50.0	50.0	58.1	58.0	1028-1040 bp	1.0	83.9
trnF-GAA-trnL-UAA_p5	TTGGATAGGATGGCCTTTGC	GGGGATATGGCGAAATTGGT	50.0	50.0	57.9	58.0	1702-1714 bp	1.0	83.6