primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CGAGGCTCCATCTATAAATGG	20	21	55.0	47.6	58.1	55.7	0.0	9.3	3.420017	122	361	287-297	1.000000	58.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCCACTGCCTTGATCCAC	CGAGGCTCCATCTATAAATGG	20	21	50.0	47.6	57.9	55.7	0.0	9.3	3.425886	122	361	300-310	1.000000	58.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CGAGGCTCCATCTATAAATGG	20	21	50.0	47.6	57.9	55.7	0.0	9.3	3.424378	122	361	301-311	1.000000	58.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CGAGGCTCCATCTATAAATGG	20	21	50.0	47.6	57.9	55.7	0.0	9.3	3.425886	122	361	302-312	1.000000	58.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CGCATGGTGGATTCACAATC	CGAGGCTCCATCTATAAATGG	20	21	50.0	47.6	57.5	55.7	6.4	9.3	3.771321	122	361	316-326	1.000000	55.1
trnF-GAA-trnL-UAA_p1	trnF-GAA-trnL-UAA	1	CATTTCTACTCTTCCCGCCC	TTTCGCGTTGGGTTAGGAAA	20	20	55.0	45.0	58.0	58.0	0.0	5.8	0.080521	13261	13682	1098-1109	1.000000	84.8
trnF-GAA-trnL-UAA_p2	trnF-GAA-trnL-UAA	2	TTTGAACTGGTGACACGAGG	TTTCGCGTTGGGTTAGGAAA	20	20	50.0	45.0	58.1	58.0	20.1	5.8	0.087343	13261	13682	792-804	1.000000	84.6
trnF-GAA-trnL-UAA_p3	trnF-GAA-trnL-UAA	3	CATTTCTACTCTTCCCGCCC	GGGGATATGGCGAAATTGGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.049697	13261	13682	1334-1345	1.000000	84.3
trnF-GAA-trnL-UAA_p4	trnF-GAA-trnL-UAA	4	TTTGAACTGGTGACACGAGG	GGGGATATGGCGAAATTGGT	20	20	50.0	50.0	58.1	58.0	20.1	0.0	0.056519	13261	13682	1028-1040	1.000000	83.9
trnF-GAA-trnL-UAA_p5	trnF-GAA-trnL-UAA	5	TTGGATAGGATGGCCTTTGC	GGGGATATGGCGAAATTGGT	20	20	50.0	50.0	57.9	58.0	3.3	0.0	0.076401	13261	13682	1702-1714	1.000000	83.6
trnT-UGU-rps4_p1	trnT-UGU-rps4	1	CTCAGAGGTTAGAGCATCGC	CTGCGGGCCTTTTACTAGTT	20	20	55.0	50.0	57.9	57.9	0.0	0.8	0.242165	14940	15225	413-430	1.000000	84.9
trnT-UGU-rps4_p2	trnT-UGU-rps4	2	CTCAGAGGTTAGAGCATCGC	AGCGCGATTGGTTTCTAAGT	20	20	55.0	45.0	57.9	57.9	0.0	12.1	0.227502	14940	15225	449-466	1.000000	84.5
trnT-UGU-rps4_p3	trnT-UGU-rps4	3	ATGGTCATCGGTTCGATTCC	CTGCGGGCCTTTTACTAGTT	20	20	50.0	50.0	57.8	57.9	0.0	0.8	0.349918	14940	15225	381-398	1.000000	84.3
trnT-UGU-rps4_p4	trnT-UGU-rps4	4	CTCAGAGGTTAGAGCATCGC	TTTGTTTGAAACGAGGCCCT	20	20	55.0	45.0	57.9	58.2	0.0	0.0	0.362446	14940	15225	365-382	1.000000	84.3
trnT-UGU-rps4_p5	trnT-UGU-rps4	5	ATGGTCATCGGTTCGATTCC	AGCGCGATTGGTTTCTAAGT	20	20	50.0	45.0	57.8	57.9	0.0	12.1	0.335256	14940	15225	417-434	1.000000	84.1
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	TGAAGGAGGCTCTTCAGGAA	21	20	47.6	50.0	57.3	58.0	0.0	15.2	1.722818	18036	18428	436-488	1.000000	73.2
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	GGCTCTTCAGGAACAGATCG	21	20	47.6	55.0	57.3	58.1	0.0	0.0	1.759544	18036	18428	429-481	1.000000	72.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	GGAGAAGCAGAAGCCCTTTT	21	20	47.6	50.0	57.3	58.1	0.0	0.0	1.776285	18036	18428	455-507	1.000000	72.6
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	TTCACTGGAGAAGCAGAAGC	21	20	47.6	50.0	57.3	57.8	0.0	0.0	1.868668	18036	18428	461-513	1.000000	71.9
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GGTTCAAATCCTATTGGACGC	AAGGAGGCTCTTCAGGAACA	21	20	47.6	50.0	57.3	58.3	0.0	15.2	1.958136	18036	18428	434-486	1.000000	71.3
rpoC2_p1	rpoC2	1	TGCCAATTCAAATGGAATGGA	GGCCCTTTAGGAATAACCGT	21	20	38.1	50.0	56.4	57.3	0.0	0.0	3.358276	25770	25889	215-215	1.000000	63.2
rpoC2_p2	rpoC2	2	TCGGGCTTATAAATTCTCTCGT	GGCCCTTTAGGAATAACCGT	22	20	40.9	50.0	57.1	57.3	0.0	0.0	3.654983	25770	25889	170-170	1.000000	62.8
rpoC2_p3	rpoC2	3	TGCCAATTCAAATGGAATGGA	GGCCCTTTAGGAATAACCGTT	21	21	38.1	47.6	56.4	58.0	0.0	0.0	3.643789	25770	25889	215-215	1.000000	60.9
rpoC2_p4	rpoC2	4	TGCCAATTCAAATGGAATGGA	AGGCCCTTTAGGAATAACCG	21	20	38.1	50.0	56.4	57.0	0.0	0.0	3.658278	25770	25889	216-216	1.000000	60.7
rpoC2_p5	rpoC2	5	TGCCAATTCAAATGGAATGGAT	GGCCCTTTAGGAATAACCGT	22	20	36.4	50.0	56.9	57.3	0.0	0.0	3.839869	25770	25889	215-215	1.000000	59.3
psaA-ycf3_p1	psaA-ycf3	1	TGCCCATTCCTCGAAAGAAG	AGCCCTTACCCCTGGTAATT	20	20	50.0	50.0	57.9	58.0	4.1	6.2	0.117992	49309	50080	718-922	1.000000	85.2
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	AGCCCTTACCCCTGGTAATT	20	20	50.0	50.0	57.9	58.0	4.1	6.2	0.119474	49309	50080	717-921	1.000000	85.2
psaA-ycf3_p3	psaA-ycf3	3	TGCCCATTCCTCGAAAGAAG	AGCTATAGCCCTTACCCCTG	20	20	50.0	55.0	57.9	58.0	4.1	21.2	0.145355	49309	50080	724-928	1.000000	84.9
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACCCCTG	20	20	50.0	55.0	57.9	58.0	4.1	21.2	0.146838	49309	50080	723-927	1.000000	84.9
psaA-ycf3_p5	psaA-ycf3	5	TGCCCATTCCTCGAAAGAAG	ATCAGGCCGCTGAATATTGG	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.212269	49309	50080	749-953	1.000000	84.3
ycf4-cemA_p1	ycf4-cemA	1	CATAGGACAGGGTGCCATTC	CGTGTTTCCGGGTATTCCTT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.130665	58386	58697	665-682	1.000000	83.2
ycf4-cemA_p2	ycf4-cemA	2	CATAGGACAGGGTGCCATTC	TCGTTGTGCGTCTTGATCAA	20	20	55.0	45.0	58.0	58.1	0.0	8.1	0.167745	58386	58697	728-745	1.000000	82.9
ycf4-cemA_p3	ycf4-cemA	3	GGGGGTCTCGAAAAACAAGT	CGTGTTTCCGGGTATTCCTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.126481	58386	58697	1062-1079	1.000000	82.1
ycf4-cemA_p4	ycf4-cemA	4	GGGGGTCTCGAAAAACAAGT	TCGTTGTGCGTCTTGATCAA	20	20	50.0	45.0	58.0	58.1	0.0	8.1	0.163561	58386	58697	1125-1142	1.000000	81.8
ycf4-cemA_p5	ycf4-cemA	5	GGGGGTCTCGAAAAACAAGT	TTCGTTGTGCGTCTTGATCA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.163561	58386	58697	1126-1143	1.000000	81.8
petA-psbJ_p1	petA-psbJ	1	CTTGTGATAGGCCCTGTTCC	TCCCTATTCATTGCGGGTTG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.095457	60523	61691	2373-2388	1.000000	82.0
petA-psbJ_p2	petA-psbJ	2	CTTGTGATAGGCCCTGTTCC	ATGTATTTGGAAGTCCCCGC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.096905	60523	61691	2324-2339	1.000000	82.0
petA-psbJ_p3	petA-psbJ	3	CTTGTGATAGGCCCTGTTCC	ATTCATTGCGGGTTGGCTAT	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.115900	60523	61691	2368-2383	1.000000	81.8
petA-psbJ_p4	petA-psbJ	4	CTTGTGATAGGCCCTGTTCC	CATTGCGGGTTGGCTATTTG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.118893	60523	61691	2365-2380	1.000000	81.8
petA-psbJ_p5	petA-psbJ	5	CTTGTGATAGGCCCTGTTCC	TCAGCACGGGTTTAGCTTAC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.133072	60523	61691	2345-2360	1.000000	81.7
petL-petG_p1	petL-petG	1	TTCGGTTTTCTACTAGCGGC	CTAACTGATCACCACGTCGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.091054	63968	64130	344-344	1.000000	81.1
petL-petG_p2	petL-petG	2	CGGTTTTCTACTAGCGGCTT	CTAACTGATCACCACGTCGG	20	20	50.0	55.0	58.0	58.1	8.3	0.0	0.096595	63968	64130	342-342	1.000000	81.1
petL-petG_p3	petL-petG	3	TCGGTTTTCTACTAGCGGCT	CTAACTGATCACCACGTCGG	20	20	50.0	55.0	58.8	58.1	8.0	0.0	0.910596	63968	64130	343-343	1.000000	74.6
petL-petG_p4	petL-petG	4	TTCGGTTTTCTACTAGCGGC	TCTAACTGATCACCACGTCG	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.914206	63968	64130	345-345	1.000000	74.5
petL-petG_p5	petL-petG	5	CGGTTTTCTACTAGCGGCTT	TCTAACTGATCACCACGTCG	20	20	50.0	50.0	58.0	57.1	8.3	0.0	0.919748	63968	64130	343-343	1.000000	74.5
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	GCTAAAGCCCCGAACCATAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.146756	64647	65087	544-567	1.000000	85.8
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	GCTAAAGCCCCGAACCATAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.148100	64647	65087	543-566	1.000000	85.8
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	GCTAAAGCCCCGAACCATAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.154792	64647	65087	555-578	1.000000	85.6
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	GCTAAAGCCCCGAACCATAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.156126	64647	65087	554-577	1.000000	85.6
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	GCTAAAGCCCCGAACCATAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.156126	64647	65087	557-580	1.000000	85.6
clpP_p1	clpP	1	GTTGGCATGGTGAGTCGTAT	CATAGAGAGCCATCGAACCG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.081679	68130	70117	2727-2795	1.000000	83.9
clpP_p2	clpP	2	GTTGGCATGGTGAGTCGTAT	ACGAACATACCCTGTCTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.091154	68130	70117	2791-2859	1.000000	83.8
clpP_p3	clpP	3	ACCTCGCCGATACTGGAATA	CATAGAGAGCCATCGAACCG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.066382	68130	70117	2902-2970	1.000000	83.7
clpP_p4	clpP	4	CTGAACGAGTCGCACATACA	CATAGAGAGCCATCGAACCG	20	20	50.0	55.0	58.0	57.9	13.6	0.0	0.080315	68130	70117	2848-2916	1.000000	83.7
clpP_p5	clpP	5	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	20	20	50.0	50.0	58.0	58.1	13.6	0.0	0.089790	68130	70117	2912-2980	1.000000	83.6
petD-rpoA_p1	petD-rpoA	1	GGTTGGGTATTGGAGCAACA	CGAGAAATCGCGGATATCGT	20	20	50.0	50.0	58.1	58.0	0.0	6.1	0.117045	75992	76209	814-820	1.000000	82.3
petD-rpoA_p2	petD-rpoA	2	TGGTTGGGTATTGGAGCAAC	CGAGAAATCGCGGATATCGT	20	20	50.0	50.0	58.1	58.0	0.0	6.1	0.117045	75992	76209	815-821	1.000000	82.3
petD-rpoA_p3	petD-rpoA	3	GTTTGATTCGTGCTTGAGCC	GAAGCCTCCCGGAATTTGAT	20	20	50.0	50.0	58.0	57.9	0.0	0.7	0.089489	75992	76209	1124-1130	1.000000	82.0
petD-rpoA_p4	petD-rpoA	4	GTTTGATTCGTGCTTGAGCC	CGAGAAATCGCGGATATCGT	20	20	50.0	50.0	58.0	58.0	0.0	6.1	0.065813	75992	76209	1318-1324	1.000000	81.8
petD-rpoA_p5	petD-rpoA	5	GTGCTTGAGCCGTATGATGA	CGAGAAATCGCGGATATCGT	20	20	50.0	50.0	58.1	58.0	0.0	6.1	0.094942	75992	76209	1309-1315	1.000000	81.6
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	106953	107527	715-716	1.000000	80.5
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	106953	107527	752-753	1.000000	79.8
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	20	20	55.0	50.0	57.8	58.1	18.9	0.0	0.311620	106953	107527	756-757	1.000000	79.6
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	AGCTCGAATTTCCCGTTGTT	20	20	55.0	45.0	57.8	58.1	18.9	0.0	0.323698	106953	107527	727-728	1.000000	79.6
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	TTTTAGGGTACTCCGGGGAA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	57.7	58.0	9.3	0.0	0.343052	106953	107527	769-770	1.000000	79.3
ycf1_p1	ycf1	1	ATCTATACGCGCTCAAAGGC	CGGGGATAACCAGAAAAGGG	20	20	50.0	55.0	58.2	57.9	5.7	0.0	0.268257	110089	110208	699-702	1.000000	83.2
ycf1_p2	ycf1	2	ACAACAAACGGATTTTCGGC	CGGGGATAACCAGAAAAGGG	20	20	45.0	55.0	57.9	57.9	0.0	0.0	0.181150	110089	110208	735-738	0.800000	74.0
ycf1_p3	ycf1	3	ACAACAAACGGATTTTCGGC	ATCTGTGCCAAGGTTTCAGG	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.213227	110089	110208	617-617	0.800000	73.1
ycf1_p4	ycf1	4	ACAACAAACGGATTTTCGGC	AAGGCGATTTCGGGGATAAC	20	20	45.0	50.0	57.9	58.0	0.0	1.7	0.165195	110089	110208	745-748	0.600000	65.5
ycf1_p5	ycf1	5	ATCTATACGCGCTCAAAGGC	AAGGCGATTTCGGGGATAAC	20	20	50.0	50.0	58.2	58.0	5.7	1.7	0.252303	110089	110208	709-712	0.600000	64.9
ycf1-rps15_p1	ycf1-rps15	1	ATTGAGCTGGAGAGGTTCCT	TCCTTTCGGTGCAATTCCAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.047721	113336	113787	1328-1340	0.800000	77.0
ycf1-rps15_p2	ycf1-rps15	2	ATTGAGCTGGAGAGGTTCCT	CGGATTTGTGACTCCCGAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060190	113336	113787	1460-1472	0.800000	76.6
ycf1-rps15_p3	ycf1-rps15	3	ATTGAGCTGGAGAGGTTCCT	TAATCGTTGCCGTTTTCCCA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.142470	113336	113787	1380-1392	0.800000	76.2
ycf1-rps15_p4	ycf1-rps15	4	ATTGAGCTGGAGAGGTTCCT	AACACCATGCATTGATGGGT	20	20	50.0	45.0	58.0	58.1	0.0	8.9	0.094638	113336	113787	1653-1665	0.800000	76.0
ycf1-rps15_p5	ycf1-rps15	5	ATTGAGCTGGAGAGGTTCCT	CCAACGTGTTACATAGGGCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.138085	113336	113787	1788-1800	0.800000	75.3
rpl32-ndhF_p1	rpl32-ndhF	1	TCCTAAGAGCAGCGTGTCTA	GGAAAAAGTAGGAGCCCCAC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.288783	123700	124181	1040-1075	1.000000	84.2
rpl32-ndhF_p2	rpl32-ndhF	2	TCCTAAGAGCAGCGTGTCTA	GTGGGAAAAAGTAGGAGCCC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.288783	123700	124181	1043-1078	1.000000	84.1
rpl32-ndhF_p3	rpl32-ndhF	3	CGAAGATCAAATGCCATGCC	GGAAAAAGTAGGAGCCCCAC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.294329	123700	124181	1097-1132	1.000000	83.9
rpl32-ndhF_p4	rpl32-ndhF	4	GAAGATCAAATGCCATGCCG	GGAAAAAGTAGGAGCCCCAC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.294329	123700	124181	1096-1131	1.000000	83.9
rpl32-ndhF_p5	rpl32-ndhF	5	GAAGATCAAATGCCATGCCG	GTGGGAAAAAGTAGGAGCCC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.294329	123700	124181	1099-1134	1.000000	83.9
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	127420	127994	715-716	1.000000	80.5
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	20	20	55.0	55.0	58.0	57.7	0.0	18.7	0.288158	127420	127994	752-753	1.000000	79.8
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.1	57.8	0.0	18.9	0.311620	127420	127994	756-757	1.000000	79.6
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	AGCTCGAATTTCCCGTTGTT	CAGAGGATTAGAGCACGTGG	20	20	45.0	55.0	58.1	57.8	0.0	18.9	0.323698	127420	127994	727-728	1.000000	79.6
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CCGTTGTTCCTCAGTAGCTC	TTTTAGGGTACTCCGGGGAA	20	20	55.0	50.0	58.0	57.7	0.0	9.3	0.343052	127420	127994	769-770	1.000000	79.3