Hotspot

ndhA

SSC · 119777-121937 · 2161 bp

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Pi 0.0074

MarkerSeek score 61.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2161
variable_sites: 16
pi: 0.0074
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCGGTTATCATAGGAAGCA	TGACGATACTCGGTAGCATAGA	45.0	45.5	56.0	58.0	2317-2324 bp	1.0	48.7
ndhA_p2	ACCGGTTATCATAGGAAGCA	ATGACGATACTCGGTAGCAT	45.0	45.0	56.0	55.9	2318-2325 bp	1.0	48.2
ndhA_p3	AACCGGTTATCATAGGAAGCA	TGACGATACTCGGTAGCATAGA	42.9	45.5	56.8	58.0	2318-2325 bp	1.0	46.9
ndhA_p4	AACCGGTTATCATAGGAAGCA	ATGACGATACTCGGTAGCAT	42.9	45.0	56.8	55.9	2319-2326 bp	1.0	46.5
ndhA_p5	ACCGGTTATCATAGGAAGCA	TGACGATACTCGGTAGCATAG	45.0	47.6	56.0	56.6	2317-2324 bp	1.0	45.1