Hotspot

rpl32-trnL-CUA

SSC · 113395-114295 · 901 bp

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Pi 0.0247
MarkerSeek score 69.20
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
901
variable_sites
22
pi
0.0247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 GGAAGGGGTATTGGACATCG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 57.7 57.9 1025-1035 bp 1.0 79.5
rpl32-trnL-CUA_p2 GGAAGGGGTATTGGACATCG AGCAGCGTGTCTACCAATTT 55.0 45.0 57.7 57.8 1018-1028 bp 1.0 79.1
rpl32-trnL-CUA_p3 GGAAGGGGTATTGGACATCG CCTAAGAGCAGCGTGTCTAC 55.0 55.0 57.7 57.8 1024-1034 bp 1.0 78.9
rpl32-trnL-CUA_p4 GGAAGGGGTATTGGACATCG CTAAGAGCAGCGTGTCTACC 55.0 55.0 57.7 57.8 1023-1033 bp 1.0 78.9
rpl32-trnL-CUA_p5 GGAAGGGGTATTGGACATCG GAGCAGCGTGTCTACCAATT 55.0 50.0 57.7 58.3 1019-1029 bp 1.0 78.3