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psaJ-rpl33

LSC · 66527-66949 · 423 bp

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Pi 0.0336

MarkerSeek score 75.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 14
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCGGTTCGTTAGCAGGTTT	ATATCTGGAAATTCCCGCGG	45.0	50.0	57.8	57.8	597-603 bp	1.0	79.2
psaJ-rpl33_p2	ATGGTTCGGTTCGTTAGCAG	ATATCTGGAAATTCCCGCGG	50.0	50.0	58.3	57.8	601-607 bp	1.0	78.9
psaJ-rpl33_p3	TTCGGTTCGTTAGCAGGTTT	AATTCCCGCGGATTCTTTCT	45.0	45.0	57.8	57.6	588-594 bp	1.0	77.4
psaJ-rpl33_p4	TTCGGTTCGTTAGCAGGTTT	ATTCCCGCGGATTCTTTCTT	45.0	45.0	57.8	57.6	587-593 bp	1.0	77.4
psaJ-rpl33_p5	GTTCGGTTCGTTAGCAGGTT	ATATCTGGAAATTCCCGCGG	50.0	50.0	58.5	57.8	598-604 bp	1.0	77.2