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psbE-petL

LSC · 63839-65194 · 1356 bp

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Pi 0.0134

MarkerSeek score 67.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1356
variable_sites: 18
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1507-1516 bp	1.0	67.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1425-1434 bp	1.0	66.9
psbE-petL_p3	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1508-1517 bp	1.0	66.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1420-1429 bp	1.0	66.6
psbE-petL_p5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	45.0	42.9	57.9	57.0	1507-1516 bp	1.0	65.5