Hotspot

atpB-rbcL

LSC · 53563-54370 · 808 bp

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Pi 0.00745

MarkerSeek score 63.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 6
pi: 0.00745
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	954-959 bp	1.0	72.2
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	953-958 bp	1.0	72.0
atpB-rbcL_p3	ATCCAGTACCGGACCAATGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	953-958 bp	1.0	71.6
atpB-rbcL_p4	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	952-957 bp	1.0	71.6
atpB-rbcL_p5	TCCAGTACCGGACCAATGAT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	951-956 bp	1.0	71.3