Hotspot

rps4-trnT-ACA

LSC · 45672-46056 · 385 bp

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Pi 0.0234

MarkerSeek score 67.70

Primer available yes

Label rps4-trnT-ACA

Feature Metadata

label_name: rps4-trnT-ACA
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 9
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-ACA_p1	CGGAGCGTGATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	563 bp	1.0	80.2
rps4-trnT-ACA_p2	CGGAGCGTGATTGGTTTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	531 bp	1.0	79.4
rps4-trnT-ACA_p3	TCTAAGATCGCTTCCTGCCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	547 bp	1.0	78.5
rps4-trnT-ACA_p4	TCTAAGATCGCTTCCTGCCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.2	57.8	515 bp	1.0	77.7
rps4-trnT-ACA_p5	CGGAGCGTGATTGGTTTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	571 bp	1.0	77.2