Hotspot

psbK-psbI

LSC · 7160-7561 · 402 bp

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Pi 0.0175

MarkerSeek score 55.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 7
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCAGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCA	50.0	31.8	57.9	54.4	455-474 bp	1.0	41.4
psbK-psbI_p2	TGGCAAGCAGCTGTAAGTTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	452-471 bp	1.0	41.4
psbK-psbI_p3	TTGGCAAGCAGCTGTAAGTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	453-472 bp	1.0	41.4
psbK-psbI_p4	TTTGGCAAGCAGCTGTAAGT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	454-473 bp	1.0	41.4
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCAT	50.0	30.4	57.9	55.0	455-474 bp	1.0	41.4