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trnK-AAA-rps16

LSC · 4304-4982 · 679 bp

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Pi 0.0162

MarkerSeek score 58.90

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 11
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	AACTCTACCGCAGATCTGGA	CGCTCAACCAACAAGAACAG	50.0	50.0	57.9	57.6	842-844 bp	1.0	76.8
trnK-AAA-rps16_p2	AAAGCCGAGTACTCTACCGT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	774-776 bp	1.0	76.7
trnK-AAA-rps16_p3	AAGCCGAGTACTCTACCGTT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.2	57.6	773-775 bp	1.0	76.7
trnK-AAA-rps16_p4	TACCGTTGAGTTAGCAACCC	CGCTCAACCAACAAGAACAG	50.0	50.0	57.5	57.6	760-762 bp	1.0	74.4
trnK-AAA-rps16_p5	AGTCGCACTTAAAAGCCGAG	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	785-787 bp	1.0	73.4