Hotspot

ndhF-rpl32

SSC · 116944-117773 · 830 bp

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Pi 0.0607

MarkerSeek score 79.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 830
variable_sites: 48
pi: 0.0607
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCGAAGATTCTTTGTTGCC	ACGAAAAAGCTTTCAACGCC	50.0	45.0	58.0	58.2	1042-1081 bp	1.0	85.2
ndhF-rpl32_p2	GCCCATATACGGCGAAGATT	ACGAAAAAGCTTTCAACGCC	50.0	45.0	57.9	58.2	1052-1091 bp	1.0	84.5
ndhF-rpl32_p3	TTAATGGTCCAAGCCCACAC	ACGAAAAAGCTTTCAACGCC	50.0	45.0	58.1	58.2	1163-1202 bp	1.0	84.0
ndhF-rpl32_p4	GGTCCAAGCCCACACATATT	ACGAAAAAGCTTTCAACGCC	50.0	45.0	57.9	58.2	1158-1197 bp	1.0	83.5
ndhF-rpl32_p5	GGCGAAGATTCTTTGTTGCC	AAGAATCCCCACCCAGGAAT	50.0	50.0	58.0	58.3	1199-1238 bp	1.0	83.3