Hotspot

rps12-clpP

LSC · 73823-73983 · 161 bp

Back to Lumnitzera

Pi 0.0186

MarkerSeek score 63.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 3
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTAGTACATGTTCCTCGGCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.9	57.9	326 bp	1.0	78.9
rps12-clpP_p2	CTAGTACATGTTCCTCGGCG	GTACAAAGAACGGGCAAACC	55.0	50.0	57.9	57.6	385 bp	1.0	76.4
rps12-clpP_p3	CCTAGTACATGTTCCTCGGC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.5	57.9	327 bp	1.0	75.7
rps12-clpP_p4	CGGGGGATTTCGTGACATTT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.5	57.9	286 bp	1.0	75.6
rps12-clpP_p5	CCTAGTACATGTTCCTCGGC	GTACAAAGAACGGGCAAACC	55.0	50.0	57.5	57.6	386 bp	1.0	73.2