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psbE-petL

LSC · 68508-69638 · 1131 bp

Back to Lumnitzera

Pi 0.00826

MarkerSeek score 61.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1131
variable_sites: 9
pi: 0.00826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	ACGCTGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1265-1278 bp	1.0	82.7
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	ACGCTGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1188-1201 bp	1.0	82.0
psbE-petL_p3	ACGAATAACCAACCCGCAAT	ACGCTGCTAGTAGAAAACCG	45.0	50.0	58.2	58.0	1274-1287 bp	1.0	81.7
psbE-petL_p4	TGCTGACGAATAACCAACCC	ACGCTGCTAGTAGAAAACCG	50.0	50.0	58.2	58.0	1279-1292 bp	1.0	81.6
psbE-petL_p5	CGAATAACCAACCCGCAATG	ACGCTGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1273-1286 bp	1.0	81.6