Hotspot

trnM-CAU-atpE

LSC · 55909-56130 · 222 bp

Back to Lumnitzera

Pi 0.0452

MarkerSeek score 78.90

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 10
pi: 0.0452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TCCGGATGAGTTCCTCTTGT	CGAGTCGAAGCTCTCAATCC	50.0	55.0	58.1	58.2	365-366 bp	1.0	80.6
trnM-CAU-atpE_p2	TCCGGATGAGTTCCTCTTGT	GAGTCGAAGCTCTCAATCCG	50.0	55.0	58.1	58.2	364-365 bp	1.0	80.6
trnM-CAU-atpE_p3	TCCGGATGAGTTCCTCTTGT	CACGAGTCGAAGCTCTCAAT	50.0	50.0	58.1	57.7	367-368 bp	1.0	79.7
trnM-CAU-atpE_p4	TCCGGATGAGTTCCTCTTGT	TAGCTGTCAGACGAGCTAGG	50.0	55.0	58.1	58.3	388-389 bp	1.0	79.1
trnM-CAU-atpE_p5	TCCGGATGAGTTCCTCTTGT	GTCGAAGCTCTCAATCCGAT	50.0	50.0	58.1	57.5	362-363 bp	1.0	77.9