Hotspot

psbZ-trnG-UCC

LSC · 38321-39007 · 687 bp

Back to Lumnitzera

Pi 0.0169

MarkerSeek score 68.30

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 11
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	TACTTGAACCTGGGCGGATA	55.0	50.0	58.0	58.1	869-877 bp	1.0	83.0
psbZ-trnG-UCC_p2	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	833-841 bp	1.0	82.9
psbZ-trnG-UCC_p3	CTTCTCCTGATGGTTGGTCG	ACATTACTTGAACCTGGGCG	55.0	50.0	58.0	58.2	873-881 bp	1.0	82.6
psbZ-trnG-UCC_p4	CCGTTGTCTTTGCTTCTCCT	TACTTGAACCTGGGCGGATA	50.0	50.0	58.1	58.1	881-889 bp	1.0	82.2
psbZ-trnG-UCC_p5	CCGTTGTCTTTGCTTCTCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	845-853 bp	1.0	82.0