Hotspot

trnR-UCU-atpA

LSC · 10417-10963 · 547 bp

Back to Lumnitzera

Pi 0.0238

MarkerSeek score 67.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 13
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	644-681 bp	1.0	67.5
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCACTGAGGAAGCAGAAGC	47.6	50.0	57.3	57.8	650-687 bp	1.0	66.7
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	CAGGAACAGATGGAACGCTT	47.6	50.0	57.3	58.2	611-648 bp	1.0	66.7
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TTCAGGAACAGATGGAACGC	47.6	50.0	57.3	58.2	613-650 bp	1.0	66.7
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	AGGAAGCAGAAGCCCTTTTG	47.6	50.0	57.3	58.4	643-680 bp	1.0	65.2