Hotspot

trnS-GCU-trnG-UCC

LSC · 8321-9100 · 780 bp

Back to Lumnitzera

Pi 0.0196

MarkerSeek score 62.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 15
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	GGATCCCTTAACTAGAGAAGCG	50.0	50.0	58.0	57.8	881-933 bp	1.0	64.6
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	GGATCCCTTAACTAGAGAAGCG	50.0	50.0	58.0	57.8	880-932 bp	1.0	64.6
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	GGATCCCTTAACTAGAGAAGCG	50.0	50.0	58.2	57.8	935-987 bp	1.0	63.3
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AGAGAAGCGATCAGTAAAACGA	50.0	40.9	58.0	57.6	868-920 bp	1.0	63.2
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	AGAGAAGCGATCAGTAAAACGA	50.0	40.9	58.0	57.6	867-919 bp	1.0	63.1