Hotspot

trnK-UUU-rps16

LSC · 4670-5574 · 905 bp

Back to Lumnitzera

Pi 0.0134

MarkerSeek score 67.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 12
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TACCGAGGGTCTGAACGAAT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1099-1100 bp	1.0	79.9
trnK-UUU-rps16_p2	ACTCTACCGAGGGTCTGAAC	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.2	58.0	1103-1104 bp	1.0	79.9
trnK-UUU-rps16_p3	CGAGGGTCTGAACGAATCTC	AAAAAGGCGCTCAACCTACA	55.0	45.0	57.8	58.0	1096-1097 bp	1.0	79.6
trnK-UUU-rps16_p4	ACAAACTCTACCGAGGGTCT	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.7	58.0	1107-1108 bp	1.0	78.7
trnK-UUU-rps16_p5	TACCGAGGGTCTGAACGAAT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	1103-1104 bp	1.0	78.3