Hotspot

rps15-ycf1

SSC · 116068-116529 · 462 bp

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Pi 0.0214
MarkerSeek score 62.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
462
variable_sites
22
pi
0.0214
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AAATAAGGAACTGCGTGGCA TGCCAATAACAGCAGTCGTT 45.0 45.0 58.1 58.1 1012-1183 bp 0.5 63.6
rps15-ycf1_p2 AAATAAGGAACTGCGTGGCA ATGCCAATAACAGCAGTCGT 45.0 45.0 58.1 57.9 1013-1184 bp 0.5 63.5
rps15-ycf1_p3 AAATAAGGAACTGCGTGGCA TCGCTATTGGTTTGATGCCA 45.0 45.0 58.1 57.9 1027-1198 bp 0.5 63.5
rps15-ycf1_p4 AAATAAGGAACTGCGTGGCA CATTGCACGACACAACAACA 45.0 45.0 58.1 57.8 1324-1495 bp 0.5 62.8
rps15-ycf1_p5 CCGTTCATAAACAGCACAGC TGCCAATAACAGCAGTCGTT 50.0 45.0 57.8 58.1 1062-1238 bp 0.5 62.4