Hotspot

psaC-ndhE

SSC · 113901-114622 · 722 bp

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Pi 0.0213
MarkerSeek score 59.70
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
722
variable_sites
31
pi
0.0213
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.66
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 AGCTCGAACACATTGGGTAC GCCATTGCTGCTGTTTAA 50.0 44.4 57.9 53.9 799-851 bp 0.5 23.4
psaC-ndhE_p2 GCTCGAACACATTGGGTACA GCCATTGCTGCTGTTTAA 50.0 44.4 58.2 53.9 798-850 bp 0.5 23.4
psaC-ndhE_p3 CAAGCTCGAACACATTGGGT GCCATTGCTGCTGTTTAA 50.0 44.4 58.8 53.9 801-853 bp 0.5 23.4
psaC-ndhE_p4 AAGCTCGAACACATTGGGTA GCCATTGCTGCTGTTTAA 45.0 44.4 57.1 53.9 800-852 bp 0.5 23.4
psaC-ndhE_p5 CGAACACATTGGGTACATCCT GCCATTGCTGCTGTTTAA 47.6 44.4 58.0 53.9 795-847 bp 0.5 23.4