Hotspot

rpl32-trnL-UAG

SSC · 111191-111801 · 611 bp

Back to Luisia

Pi 0.0107
MarkerSeek score 59.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
611
variable_sites
13
pi
0.0107
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAAGTTTCTTTGTTGCACA TCCTAAGAGCAGCGTGTCTA 30.0 50.0 52.6 57.9 628-701 bp 1.0 45.5
rpl32-trnL-UAG_p2 AAAGTTTCTTTGTTGCACAA TCCTAAGAGCAGCGTGTCTA 30.0 50.0 52.6 57.9 627-700 bp 1.0 45.5
rpl32-trnL-UAG_p3 AAGTTTCTTTGTTGCACAAA TCCTAAGAGCAGCGTGTCTA 30.0 50.0 52.6 57.9 626-699 bp 1.0 45.5
rpl32-trnL-UAG_p4 AGTTTCTTTGTTGCACAAAA TCCTAAGAGCAGCGTGTCTA 30.0 50.0 52.6 57.9 625-698 bp 1.0 45.5
rpl32-trnL-UAG_p5 AGTTTCTTTGTTGCACAAAA AGCAGCGTGTCTACCAATTT 30.0 45.0 52.6 57.8 618-691 bp 1.0 45.5